School of Science - Te Kura Pūtaiao

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https://hdl.handle.net/10292/1333
Research at AUT's School of Science - Te Kura Pūtaiao is focused on key scientific issues with regional and global significance. The common theme connecting all research areas is sustainability – in the broadest sense as it relates to environmental and human health. Our research is closely allied to teaching and learning opportunities at undergraduate and postgraduate level within the school. Research is organised in five main areas:

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Browsing School of Science - Te Kura Pūtaiao by Subject "0301 Analytical Chemistry"

Now showing 1 - 3 of 3
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    Geometric Implications of Photodiode Arrays on Received Power Distribution in Mobile Underwater Optical Wireless Communication
    (MDPI AG, 2024-05-28) Govinda Waduge, Tharuka; Seet, Boon-Chong; Vopel, Kay
    Underwater optical wireless communication (UOWC) has gained interest in recent years with the introduction of autonomous and remotely operated mobile systems in blue economic ventures such as offshore food production and energy generation. Here, we devised a model for estimating the received power distribution of diffused line-of-sight mobile optical links, accommodating irregular intensity distributions beyond the beam-spread angle of the emitter. We then used this model to conduct a spatial analysis investigating the parametric influence of the placement, orientation, and angular spread of photodiodes in array-based receivers on the mobile UOWC links in different Jerlov seawater types. It revealed that flat arrays were best for links where strict alignment could be maintained, whereas curved arrays performed better spatially but were not always optimal. Furthermore, utilizing two or more spectrally distinct wavelengths and more bandwidth-efficient modulation may be preferred for received-signal intensity-based localization and improving link range in clearer oceans, respectively. Considering the geometric implications of the array of receiver photodiodes for mobile UOWCs, we recommend the use of dynamically shape-shifting array geometries.
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    Metabolic Regulation of Copper Toxicity during Marine Mussel Embryogenesis
    (MDPI AG, 2023-07-11) Young, Tim; Gale, Samantha L; Ragg, Norman LC; Sander, Sylvia G; Burritt, David J; Benedict, Billy; Le, Dung V; Villas-Bôas, Silas G; Alfaro, Andrea C
    The development of new tools for assessing the health of cultured shellfish larvae is crucial for aquaculture industries to develop and refine hatchery methodologies. We established a large-volume ecotoxicology/health stressor trial, exposing mussel (Perna canaliculus) embryos to copper in the presence of ethylenediaminetetraacetic acid (EDTA). GC/MS-based metabolomics was applied to identify potential biomarkers for monitoring embryonic/larval health and to characterise mechanisms of metal toxicity. Cellular viability, developmental abnormalities, larval behaviour, mortality, and a targeted analysis of proteins involved in the regulation of reactive oxygen species were simultaneously evaluated to provide a complementary framework for interpretative purposes and authenticate the metabolomics data. Trace metal analysis and speciation modelling verified EDTA as an effective copper chelator. Toxicity thresholds for P. canaliculus were low, with 10% developmental abnormalities in D-stage larvae being recorded upon exposure to 1.10 μg·L-1 bioavailable copper for 66 h. Sublethal levels of bioavailable copper (0.04 and 1.10 μg·L-1) caused coordinated fluctuations in metabolite profiles, which were dependent on development stage, treatment level, and exposure duration. Larvae appeared to successfully employ various mechanisms involving the biosynthesis of antioxidants and a restructuring of energy-related metabolism to alleviate the toxic effects of copper on cells and developing tissues. These results suggest that regulation of trace metal-induced toxicity is tightly linked with metabolism during the early ontogenic development of marine mussels. Lethal-level bioavailable copper (50.3 μg·L-1) caused severe metabolic dysregulation after 3 h of exposure, which worsened with time, substantially delayed embryonic development, induced critical oxidative damage, initiated the apoptotic pathway, and resulted in cell/organism death shortly after 18 h of exposure. Metabolite profiling is a useful approach to (1) assess the health status of marine invertebrate embryos and larvae, (2) detect early warning biomarkers for trace metal contamination, and (3) identify novel regulatory mechanisms of copper-induced toxicity.
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    Metabolite Changes of Perna canaliculus Following a Laboratory Marine Heatwave Exposure: Insights from Metabolomic Analyses
    (MDPI AG, 2023-07-03) Azizan, Awanis; Venter, Leonie; Jansen van Rensburg, Peet J; Ericson, Jessica A; Ragg, Norman LC; Alfaro, Andrea C
    Temperature is considered to be a major abiotic factor influencing aquatic life. Marine heatwaves are emerging as threats to sustainable shellfish aquaculture, affecting the farming of New Zealand's green-lipped mussel [Perna canaliculus (Gmelin, 1791)]. In this study, P. canaliculus were gradually exposed to high-temperature stress, mimicking a five-day marine heatwave event, to better understand the effects of heat stress on the metabolome of mussels. Following liquid chromatography-tandem mass spectrometry analyses of haemolymph samples, key sugar-based metabolites supported energy production via the glycolysis pathway and TCA cycle by 24 h and 48 h of heat stress. Anaerobic metabolism also fulfilled the role of energy production. Antioxidant molecules acted within thermally stressed mussels to mitigate oxidative stress. Purine metabolism supported tissue protection and energy replenishment. Pyrimidine metabolism supported the protection of nucleic acids and protein synthesis. Amino acids ensured balanced intracellular osmolality at 24 h and ammonia detoxification at 48 h. Altogether, this work provides evidence that P. canaliculus has the potential to adapt to heat stress up to 24 °C by regulating its energy metabolism, balancing nucleotide production, and implementing oxidative stress mechanisms over time. The data reported herein can also be used to evaluate the risks of heatwaves and improve mitigation strategies for aquaculture.
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