The Impact of Curcumin-graphene Based Nanoformulation on Cellular Interaction and Redox-activated Apoptosis: An in Vitro Colon Cancer Study

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aut.relation.articlenumbere05360en_NZ
aut.relation.issue11en_NZ
aut.relation.journalHeliyonen_NZ
aut.relation.volume6en_NZ
aut.researcherDrabsch, Julie
dc.contributor.authorAl-Ani, LAen_NZ
dc.contributor.authorKadir, FAen_NZ
dc.contributor.authorHashim, NMen_NZ
dc.contributor.authorJulkapli, NMen_NZ
dc.contributor.authorSeyfoddin, Aen_NZ
dc.contributor.authorLu, Jen_NZ
dc.contributor.authorAlSaadi, MAen_NZ
dc.contributor.authorYehye, WAen_NZ
dc.date.accessioned2020-12-14T01:50:26Z
dc.date.available2020-12-14T01:50:26Z
dc.date.copyright2020en_NZ
dc.date.issued2020en_NZ
dc.description.abstractNatural plants derivatives have gained enormous merits in cancer therapy applications upon formulation with nanomaterials. Curcumin, as a popular research focus has acquired such improvements surpassing its disadvantageous low bioavailability. To this point, the available research data had confirmed the importance of nanomaterial type in orienting cellular response and provoking different toxicological and death mechanisms that may range from physical membrane damage to intracellular changes. This in turn underlines the poorly studied field of nanoformulation interaction with cells as the key determinant in toxicology outcomes. In this work, curcumin-AuNPs-reduced graphene oxide nanocomposite (CAG) was implemented as a model, to study the impact on cellular membrane integrity and the possible redox changes using colon cancer in vitro cell lines (HT-29 and SW-948), representing drug-responsive and resistant subtypes. Morphological and biochemical methods of transmission electron microscopy (TEM), apoptosis assay, reactive oxygen species (ROS) and antioxidants glutathione and superoxide dismutase (GSH and SOD) levels were examined with consideration to suitable protocols and vital optimizations. TEM micrographs proved endocytic uptake with succeeding cytoplasm deposition, which unlike other nanomaterials studied previously, conserved membrane integrity allowing intracellular cytotoxic mechanism. Apoptosis was confirmed with gold-standard morphological features observed in micrographs, while redox parameters revealed a time-dependent increase in ROS accompanied with regressive GSH and SOD levels. Collectively, this work demonstrates the success of graphene as a platform for curcumin intracellular delivery and cytotoxicity, and further highlights the importance of suitable in vitro methods to be used for nanomaterial validation.en_NZ
dc.identifier.citationHeliyon, 6(11), e05360.
dc.identifier.doi10.1016/j.heliyon.2020.e05360en_NZ
dc.identifier.issn2405-8440en_NZ
dc.identifier.urihttps://hdl.handle.net/10292/13876
dc.publisherElsevier
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S2405844020322039?via%3Dihub
dc.rights© 2020 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/).
dc.rights.accessrightsOpenAccessen_NZ
dc.subjectCancer research; Cell biology; Nanotechnology; Natural products; Anticancer; Cell membrane; Oxidative stress; Apoptosis
dc.titleThe Impact of Curcumin-graphene Based Nanoformulation on Cellular Interaction and Redox-activated Apoptosis: An in Vitro Colon Cancer Studyen_NZ
dc.typeJournal Article
pubs.elements-id393946
pubs.organisational-data/AUT
pubs.organisational-data/AUT/Health & Environmental Science
pubs.organisational-data/AUT/Health & Environmental Science/Applied Science
pubs.organisational-data/AUT/Health & Environmental Science/School of Science
pubs.organisational-data/AUT/PBRF
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences/HI Interprofessional 2018 PBRF
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