Host Individual and Gut Location Are More Important in Gut Microbiota Community Composition Than Temporal Variation in the Marine Herbivorous Fish Kyphosus sydneyanus

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aut.relation.articlenumber275
aut.relation.issue1
aut.relation.journalBMC Microbiology
aut.relation.volume23
dc.contributor.authorPisaniello, Alessandro
dc.contributor.authorHandley, Kim M
dc.contributor.authorWhite, W Lindsey
dc.contributor.authorAngert, Esther R
dc.contributor.authorBoey, Jian Sheng
dc.contributor.authorClements, Kendall D
dc.date.accessioned2023-10-02T22:15:31Z
dc.date.available2023-10-02T22:15:31Z
dc.date.issued2023-09-29
dc.description.abstractBackground Gut microbiota play a key role in the nutrition of many marine herbivorous fishes through hindgut fermentation of seaweed. Gut microbiota composition in the herbivorous fish Kyphosus sydneyanus (family Kyphosidae) varies between individuals and gut sections, raising two questions: (i) is community composition stable over time, especially given seasonal shifts in storage metabolites of dietary brown algae, and (ii) what processes influence community assembly in the hindgut? Results We examined variation in community composition in gut lumen and mucosa samples from three hindgut sections of K. sydneyanus collected at various time points in 2020 and 2021 from reefs near Great Barrier Island, New Zealand. 16S rRNA gene analysis was used to characterize microbial community composition, diversity and estimated density. Differences in community composition between gut sections remained relatively stable over time, with little evidence of temporal variation. Clostridia dominated the proximal hindgut sections and Bacteroidia the most distal section. Differences were detected in microbial composition between lumen and mucosa, especially at genus level. Conclusions High variation in community composition and estimated bacterial density among individual fish combined with low variation in community composition temporally suggests that initial community assembly involved environmental selection and random sampling/neutral effects. Community stability following colonisation could also be influenced by historical contingency, where early colonizing members of the community may have a selective advantage. The impact of temporal changes in the algae may be limited by the dynamics of substrate depletion along the gut following feeding, i.e. the depletion of storage metabolites in the proximal hindgut. Estimated bacterial density, showed that Bacteroidota has the highest density (copies/mL) in distal-most lumen section V, where SCFA concentrations are highest. Bacteroidota genera Alistipes and Rikenella may play important roles in the breakdown of seaweed into useful compounds for the fish host.
dc.identifier.citationBMC Microbiology, ISSN: 1471-2180 (Online), Springer Science and Business Media LLC, 23(1). doi: 10.1186/s12866-023-03025-2
dc.identifier.doi10.1186/s12866-023-03025-2
dc.identifier.issn1471-2180
dc.identifier.urihttp://hdl.handle.net/10292/16734
dc.languageen
dc.publisherSpringer Science and Business Media LLC
dc.relation.urihttps://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-023-03025-2
dc.rights.accessrightsOpenAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject06 Biological Sciences
dc.subject07 Agricultural and Veterinary Sciences
dc.subject11 Medical and Health Sciences
dc.subjectMicrobiology
dc.subject3107 Microbiology
dc.subject3207 Medical microbiology
dc.titleHost Individual and Gut Location Are More Important in Gut Microbiota Community Composition Than Temporal Variation in the Marine Herbivorous Fish Kyphosus sydneyanus
dc.typeJournal Article
pubs.elements-id525157
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