Covalently Immobilized Battacin Lipopeptide Gels With Activity Against Bacterial Biofilms

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aut.relation.articlenumber5945en_NZ
aut.relation.issue24en_NZ
aut.relation.journalMoleculesen_NZ
aut.relation.volume25en_NZ
aut.researcherDrabsch, Julie
dc.contributor.authorDe Zoysa, GHen_NZ
dc.contributor.authorWang, Ken_NZ
dc.contributor.authorLu, Jen_NZ
dc.contributor.authorHemar, Yen_NZ
dc.contributor.authorSarojini, Ven_NZ
dc.date.accessioned2021-02-09T01:13:53Z
dc.date.available2021-02-09T01:13:53Z
dc.date.copyright2020en_NZ
dc.date.issued2020en_NZ
dc.description.abstractNovel antibiotic treatments are in increasing demand to tackle life-threatening infections from bacterial pathogens. In this study, we report the use of a potent battacin lipopeptide as an antimicrobial gel to inhibit planktonic and mature biofilms of S. aureus and P. aeruginosa. The antimicrobial gels were made by covalently linking the N-terminal cysteine containing lipopeptide (GZ3.163) onto the polyethylene glycol polymer matrix and initiating gelation using thiol-ene click chemistry. The gels were prepared both in methanol and in water and were characterised using rheology, Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM). Antibacterial and antibiofilm analyses revealed that the gels prepared in methanol have better antibacterial and antibiofilm activity. Additionally, a minimum peptide content of 0.5 wt% (relative to polymer content) is required to successfully inhibit the planktonic bacterial growth and disperse mature biofilms of P. aeruginosa and S. aureus. The antibacterial activity of these lipopeptide gels is mediated by a contact kill mechanism of action. The gels are non-haemolytic against mouse red blood cells and are non-cytotoxic against human dermal fibroblasts. Findings from this study show that battacin lipopeptide gels have the potential to be developed as novel topical antibacterial agents to combat skin infections, particularly caused by S. aureus.en_NZ
dc.identifier.citationMolecules, 25(24), 5945. doi:10.3390/molecules25245945
dc.identifier.doi10.3390/molecules25245945en_NZ
dc.identifier.issn1420-3049en_NZ
dc.identifier.urihttps://hdl.handle.net/10292/13975
dc.languageengen_NZ
dc.publisherMDPI AGen_NZ
dc.relation.urihttps://www.mdpi.com/1420-3049/25/24/5945
dc.rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
dc.rights.accessrightsOpenAccessen_NZ
dc.subjectAntimicrobial hydrogelsen_NZ
dc.subjectAntimicrobial lipopeptidesen_NZ
dc.subjectBacterial biofilm inhibitionen_NZ
dc.subjectNon haemolyticen_NZ
dc.subjectNon-cytotoxicen_NZ
dc.titleCovalently Immobilized Battacin Lipopeptide Gels With Activity Against Bacterial Biofilmsen_NZ
dc.typeJournal Article
pubs.elements-id396402
pubs.organisational-data/AUT
pubs.organisational-data/AUT/Health & Environmental Science
pubs.organisational-data/AUT/PBRF
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences/HI Interprofessional 2018 PBRF
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