Metabolite Changes of Perna canaliculus Following a Laboratory Marine Heatwave Exposure: Insights from Metabolomic Analyses

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aut.relation.issue7
aut.relation.journalMetabolites
aut.relation.startpage815
aut.relation.volume13
dc.contributor.authorAzizan, Awanis
dc.contributor.authorVenter, Leonie
dc.contributor.authorJansen van Rensburg, Peet J
dc.contributor.authorEricson, Jessica A
dc.contributor.authorRagg, Norman LC
dc.contributor.authorAlfaro, Andrea C
dc.date.accessioned2023-08-09T22:57:58Z
dc.date.available2023-08-09T22:57:58Z
dc.date.issued2023-07-03
dc.description.abstractTemperature is considered to be a major abiotic factor influencing aquatic life. Marine heatwaves are emerging as threats to sustainable shellfish aquaculture, affecting the farming of New Zealand's green-lipped mussel [Perna canaliculus (Gmelin, 1791)]. In this study, P. canaliculus were gradually exposed to high-temperature stress, mimicking a five-day marine heatwave event, to better understand the effects of heat stress on the metabolome of mussels. Following liquid chromatography-tandem mass spectrometry analyses of haemolymph samples, key sugar-based metabolites supported energy production via the glycolysis pathway and TCA cycle by 24 h and 48 h of heat stress. Anaerobic metabolism also fulfilled the role of energy production. Antioxidant molecules acted within thermally stressed mussels to mitigate oxidative stress. Purine metabolism supported tissue protection and energy replenishment. Pyrimidine metabolism supported the protection of nucleic acids and protein synthesis. Amino acids ensured balanced intracellular osmolality at 24 h and ammonia detoxification at 48 h. Altogether, this work provides evidence that P. canaliculus has the potential to adapt to heat stress up to 24 °C by regulating its energy metabolism, balancing nucleotide production, and implementing oxidative stress mechanisms over time. The data reported herein can also be used to evaluate the risks of heatwaves and improve mitigation strategies for aquaculture.
dc.identifier.citationMetabolites, ISSN: 2218-1989 (Print); 2218-1989 (Online), MDPI AG, 13(7), 815-. doi: 10.3390/metabo13070815
dc.identifier.doi10.3390/metabo13070815
dc.identifier.issn2218-1989
dc.identifier.issn2218-1989
dc.identifier.urihttp://hdl.handle.net/10292/16528
dc.languageeng
dc.publisherMDPI AG
dc.relation.urihttps://www.mdpi.com/2218-1989/13/7/815
dc.rights.accessrightsOpenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectNew Zealand
dc.subjectPerna canaliculus
dc.subjectaquaculture
dc.subjectmarine heatwave
dc.subjectmetabolomics
dc.subjectmussels
dc.subjectpathways
dc.subjectstress
dc.subjecttemperature
dc.subject3205 Medical Biochemistry and Metabolomics
dc.subject32 Biomedical and Clinical Sciences
dc.subject14 Life Below Water
dc.subject7 Affordable and Clean Energy
dc.subject0301 Analytical Chemistry
dc.subject0601 Biochemistry and Cell Biology
dc.subject1103 Clinical Sciences
dc.subject3101 Biochemistry and cell biology
dc.subject3205 Medical biochemistry and metabolomics
dc.subject3401 Analytical chemistry
dc.titleMetabolite Changes of Perna canaliculus Following a Laboratory Marine Heatwave Exposure: Insights from Metabolomic Analyses
dc.typeJournal Article
pubs.elements-id519521
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