Interactive Effects of Elevated Temperature and Photobacterium swingsii Infection on the Survival and Immune Response of Marine Mussels (Perna canaliculus): A Summer Mortality Scenario

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aut.relation.articlenumber106392
aut.relation.journalMar Environ Res
aut.relation.startpage106392
aut.relation.volume196
dc.contributor.authorAzizan, Awanis
dc.contributor.authorVenter, Leonie
dc.contributor.authorZhang, Jingjing
dc.contributor.authorYoung, Tim
dc.contributor.authorEricson, Jessica A
dc.contributor.authorDelorme, Natalí J
dc.contributor.authorRagg, Norman LC
dc.contributor.authorAlfaro, Andrea C
dc.date.accessioned2024-02-20T03:42:10Z
dc.date.available2024-02-20T03:42:10Z
dc.date.issued2024-02-03
dc.description.abstractThe New Zealand Greenshell™ mussel (Perna canaliculus) is an economically important aquaculture species. Prolonged increases in seawater temperature above mussel thermotolerance ranges pose a significant threat to mussel survival and health, potentially increasing susceptibility to bacterial infections. Using challenge experiments, this study examined the combined effects of increased seawater temperature and bacterial (Photobacterium swingsii) infection on animal survival, haemocyte and biochemical responses of adult mussels. Mussels maintained at three temperatures (16, 20 and 24 °C) for seven days were either not injected (control), injected with sterile marine broth (injection control) or P. swingsii (challenged with medium and high doses) and monitored daily for five days. Haemolymph and tissue samples were collected at 24, 48, 72, 96, 120 h post-challenge and analysed to quantify bacterial colonies, haemocyte responses and biochemical responses. Mussels infected with P. swingsii exhibited mortalities at 20 and 24 °C, likely due to a compromised immune system, but no mortalities were observed when temperature was the only stressor. Bacterial colony counts in haemolymph decreased over time, suggesting bacterial clearance followed by the activation of immune signalling pathways. Total haemocyte counts and viability data supports haemocyte defence functions being stimulated in the presence of high pathogen loads at 24 °C. In the gill tissue, oxidative stress responses, measured as total antioxidant capacity and malondialdehyde (MDA) levels, were higher in infected mussels (compared to the controls) after 24h and 120h post-challenge at the lowest (16 °C) and highest temperatures (24 °C), indicating the presence of oxidative stress due to temperature and pathogen stressors. Overall, this work confirms that Photobacterium swingsii is pathogenic to P. canaliculus and indicates that mussels may be more vulnerable to bacterial pathogens under conditions of elevated temperature, such as those predicted under future climate change scenarios.
dc.identifier.citationMar Environ Res, ISSN: 0141-1136 (Print); 1879-0291 (Online), Elsevier BV, 196, 106392-. doi: 10.1016/j.marenvres.2024.106392
dc.identifier.doi10.1016/j.marenvres.2024.106392
dc.identifier.issn0141-1136
dc.identifier.issn1879-0291
dc.identifier.urihttp://hdl.handle.net/10292/17241
dc.languageeng
dc.publisherElsevier BV
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S0141113624000539
dc.rights© 2024 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by nc/4.0/).
dc.rights.accessrightsOpenAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectBacteria
dc.subjectBiomarker
dc.subjectGreenshell™ mussel
dc.subjectImmune response
dc.subjectMultiple stresses
dc.subjectPerna canaliculus
dc.subjectPhotobacterium
dc.subjectSummer mortality
dc.subjectTemperature
dc.subject31 Biological Sciences
dc.subject41 Environmental Sciences
dc.subject34 Chemical Sciences
dc.subjectInfectious Diseases
dc.subjectVaccine Related
dc.subjectEmerging Infectious Diseases
dc.subject2.2 Factors relating to the physical environment
dc.subject2 Aetiology
dc.subjectInfection
dc.subject14 Life Below Water
dc.subject3 Good Health and Well Being
dc.subject03 Chemical Sciences
dc.subject05 Environmental Sciences
dc.subject06 Biological Sciences
dc.subjectMarine Biology & Hydrobiology
dc.subject31 Biological sciences
dc.subject34 Chemical sciences
dc.subject41 Environmental sciences
dc.titleInteractive Effects of Elevated Temperature and Photobacterium swingsii Infection on the Survival and Immune Response of Marine Mussels (Perna canaliculus): A Summer Mortality Scenario
dc.typeJournal Article
pubs.elements-id538811
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